10^th European Nephrology Conference

Biography

Biography: Daniel Schock Kusch

Abstract

Transcutaneous measurement of glomerular filtration rate (_tGFR) is now frequently used in preclinical in vivo animal studies. _tGFR allows consecutive measurements on the same animal, including multiple measurements on a daily basis. A description of the measurement device and its many applications, along with examples from the recent literature will be given. We will highlight the fields of interest in which the system is used and give an overview about its performance versus endogenous and other exogenous methods of GFR measurement. A special focus will be put on the precision of _tGFR compared to standard measurements employed in the research setting. A novel kinetic model for the description of transcutaneously measured excretion kinetics of the fluorescent GFR tracer FITC-Sinistrin was recently described. Using this new kinetic model designated _tGFR_3cp.b.m), _tGFR measurements in the rat model reached comparable precision as GFR measurements assessed using a gold standard technique based on constant infusion (cGFR). The precision of _tGFR assessment when using _tGFR_3cp.b.m, coupled with the capability of consecutive GFR measurements in the same animal over a long time span should enhance the quality of, and reduce the cost of, preclinical assessment of renal function in pharmaceutical research.