Nephrology & Therapeutics

Biography

Biography: Mohamed Siyab Eldin Elsadig Ahmed

Abstract

Anemia is a major complication of end stage renal disease. It is mainly the result of impaired formation of erythrocytes due to lack of erythropoietin and iron deficiency. However, compelling evidence points to the contribution of accelerated erythrocyte death, which decreases the life span of circulating erythrocytes. Erythrocytes may enter suicidal death or eryptosis, which is characterized by cell shrinkage and by cell membrane scrambling with phosphatidylserine-exposure at the erythrocyte surface. Triggers of eryptosis include increase of cytosolic Ca²⁺-activity ([Ca²⁺]i). Erythrocytes could be sensitized to cytosolic Ca²⁺ by ceramide. In end stage renal disease, eryptosis may possibly be stimulated by uremic toxins. The present study explored, whether the uremic toxin acrolein could trigger eryptosis.

Cell volume was estimated from forward scatter, phosphatidylserine exposure from annexin-V-binding, hemolysis from hemoglobin release, [Ca²⁺]i from Fluo3-fluorescence, and ceramide from fluorescent antibodies.

In results a 48 h exposure to acrolein (30 – 50 μM) did not significantly modify [Ca²⁺]i but significantly decreased forward scatter and increased annexin-V-binding. Acrolein further triggered slight, but significant hemolysis and increased ceramide formation in erythrocytes. Acrolein (50 μM) induced annexin-V-binding was significantly blunted in the nominal absence of extracellular Ca²⁺. Acrolein augmented the annexin-V-binding following treatment with Ca²⁺ ionophore ionomycin (1 μM).

Finally we concluded that acrolein stimulates suicidal erythrocyte death
or eryptosis, an effect at least in part due to stimulation of ceramide formation with subsequent sensitisation of the erythrocytes to cytosolic Ca²⁺.